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Part:BBa_K784020

Designed by: Hila Korach Rechtman   Group: iGEM12_Technion   (2012-09-16)

Phage Lambda fragment 6 - regulation

This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation.
Each fragment was inserted into pSB1C3_MCS plasmid (BBa_K784023), which was designed mainly for this purpose. The MCS contain PacI and SpeI recognition sites, which does not appear at the phage genome.
More information is available [http://2012.igem.org/Team:Technion/Project/Phage here].
Fragment 6 contains all the regulation sequences, including the phages PR and PL promoters, the OR and OL operators, and the coding regions for the regulatory proteins N, CI and CII.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3822
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1500
    Illegal PstI site found at 3822
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2532
    Illegal BglII site found at 4924
    Illegal BglII site found at 5575
    Illegal BglII site found at 5635
    Illegal BamHI site found at 1320
    Illegal XhoI site found at 319
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3822
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3822
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1148
    Illegal SapI.rc site found at 1621


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